Thermo Fisher Scientific RNF144B Monoclonal Antibody (OTI9H10)
RNF144B 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC/IF, Flow cytometry에 적합. Human 시료에 반응하며, Affinity Chromatography로 정제된 액상 형태. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9H10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–256 of RNF144B produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723589 |
Target Information
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation.
Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism.
However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; but, it is not an anti-apoptotic protein per se.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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배송 정보
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