Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), PE, eBioscience
인간 CD8a를 인식하는 단일클론 항체로, PE 형광표지되어 Flow Cytometry 분석에 최적화됨. CD8α/β 및 CD8α/α 발현 세포 검출에 사용. 0.25 µg/test 권장, 4°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 47 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RPA-T8 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657771 |
Additional Available Formats
- Alexa Fluor 532, 561, 660, 700
- APC, APC-eFluor 780
- Brilliant UV 395~805
- Brilliant Violet 421~786
- eFluor 450, 506
- FITC
- NovaFluor Blue, Red, Yellow series
- PE-Cyanine5, 5.5, 7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436~780
- Request custom conjugation
Product Specific Information
Description:
The RPA-T8 monoclonal antibody reacts with the human CD8a molecule (~32–34 kDa), expressed as a heterodimer (CD8α/β) or homodimer (CD8α/α). CD8a is found on most thymocytes, a subset of mature T cells, and NK cells. It binds MHC class I and associates with p56lck kinase, playing a role in T-cell development and activation.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometry using normal human peripheral blood cells. Recommended ≤0.25 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best performance.
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It plays a key role in T cell signal transduction, differentiation, and activation. CD8 is mainly expressed on T cells as a disulfide-linked heterodimer (CD8α/β), functioning as a co-receptor for MHC class I molecules.
CD8α is essential for MHC-I binding and is also found on subsets of T cells, NK cells, monocytes, and dendritic cells as homodimers (CD8α/α).
Upon MHC-I/peptide complex ligation, CD8 recruits Lck kinase, promoting lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation. Activated CTLs are crucial for pathogen and tumor cell clearance. Differentiation into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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