Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), PE, eBioscience

Applications and Tested Dilution

• Publications: 13 (Flow Cytometry), 1 (Miscellaneous PubMed)

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Custom conjugation available upon request

Product Specific Information

The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule.
CD8 beta associates with CD8 alpha to form the CD8α/β heterodimer, expressed on most thymocytes and peripheral cytotoxic αβ T cells.
CD8 binds to MHC class I and plays a crucial role in T cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤0.125 µg/test (in 100 µL final volume, 10⁵–10⁸ cells/test recommended)
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD8B antigen is a cell surface glycoprotein expressed on most cytotoxic T lymphocytes, mediating cell-cell interactions in the immune system.
It functions as a coreceptor with the T-cell receptor to recognize antigens presented by class I MHC molecules.
The CD8 complex can be a homodimer (αα) or heterodimer (αβ), with both chains showing homology to immunoglobulin variable light chains.
This gene encodes the beta chain isoforms, with multiple alternatively spliced variants and a pseudogene on chromosome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지