Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), PE, eBioscience
Thermo Fisher eBioKMC8 CD9 단일클론 항체는 마우스 CD9 검출용으로 개발된 PE 결합 항체입니다. Flow cytometry 및 면역세포 분석에 적합하며, 0.5 µg/test로 최적화되어 있습니다. 4°C 보관, 동결 금지. 연구용 전용 제품입니다.
- 카탈로그번호
- 12-0091-81
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), PE, eBioscience
Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 14 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioKMC8 (KMC8) |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_891498 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- APC
- eFluor 450
- FITC
- Super Bright 600
- Request custom conjugation
Product Specific Information
The eBioKMC8 monoclonal antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily. CD9 is expressed by multiple cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts, and neuroblasts.
On T cells, CD9 acts as a co-stimulatory molecule and is also expressed in oocytes, where deficiency causes sterility due to defective gamete fusion. In mouse macrophages, CD9 associates with FcgammaRs to modify phagocytosis and inflammatory signals. In B cells, CD9 serves as a marker for marginal zone B cells, B1 cells, and plasma cells.
CD9 facilitates association of heterologous MHC II molecules in dendritic cells. Expression levels may vary between donors.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes and bone marrow. Use ≤ 0.5 µg/test (100 µL final volume). Optimize cell number empirically (10⁵–10⁸ cells/test).
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD9 antigen is a glycoprotein expressed on developing B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated T lymphocytes, neurons, and glial cells. It belongs to the tetraspanin family that spans the membrane four times.
In pre-B cells and platelets, CD9 regulates activation and aggregation through association with integrin CD41/CD61 (GPIIb/GPIIIa).
CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion. It plays roles in cell adhesion and proliferation and is essential for MHC II molecule association on dendritic cells for effective T cell stimulation.
CD9 expression is linked to suppression of cancer cell motility and metastasis and is considered a metastasis suppressor in solid tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.