Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), PE, eBioscience

Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), PE, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• eFluor 450
• FITC
• Super Bright 600
• Request custom conjugation

Product Specific Information

The eBioKMC8 monoclonal antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily. CD9 is expressed by multiple cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts, and neuroblasts.
On T cells, CD9 acts as a co-stimulatory molecule and is also expressed in oocytes, where deficiency causes sterility due to defective gamete fusion. In mouse macrophages, CD9 associates with FcgammaRs to modify phagocytosis and inflammatory signals. In B cells, CD9 serves as a marker for marginal zone B cells, B1 cells, and plasma cells.
CD9 facilitates association of heterologous MHC II molecules in dendritic cells. Expression levels may vary between donors.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes and bone marrow. Use ≤ 0.5 µg/test (100 µL final volume). Optimize cell number empirically (10⁵–10⁸ cells/test).
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD9 antigen is a glycoprotein expressed on developing B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated T lymphocytes, neurons, and glial cells. It belongs to the tetraspanin family that spans the membrane four times.
In pre-B cells and platelets, CD9 regulates activation and aggregation through association with integrin CD41/CD61 (GPIIb/GPIIIa).
CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion. It plays roles in cell adhesion and proliferation and is essential for MHC II molecule association on dendritic cells for effective T cell stimulation.
CD9 expression is linked to suppression of cancer cell motility and metastasis and is considered a metastasis suppressor in solid tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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