Thermo Fisher Scientific DDB1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:10,000
• Publications: View 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000
• Publications: View publications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:1,000
• Publications: View publications

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, A549, H1299, HCT116, MCF-7, Neuro2A, GL261, C8D30, PC-12
• Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Bovine (100%)
• IHC notes: Requires antigen retrieval using heat-mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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