Thermo Fisher Scientific DDB1 Polyclonal Antibody
Thermo Fisher Scientific의 DDB1 Polyclonal Antibody는 Human, Mouse, Rat에 반응하며 Western Blot, IHC, ICC/IF에 사용 가능합니다. 항원 친화 크로마토그래피로 정제된 액상 항체로, 1 mg/mL 농도이며 장기 보관 시 -20°C에서 보관합니다. 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:10,000
- Publications: View 1 publication
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100–1:1,000
- Publications: View publications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:1,000
- Publications: View publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 1078–1140 of DDB1 (Uniprot ID#Q16531) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2551983 |
Product Specific Information
- Recommended positive controls: 293T, A549, H1299, HCT116, MCF-7, Neuro2A, GL261, C8D30, PC-12
- Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Bovine (100%)
- IHC notes: Requires antigen retrieval using heat-mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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