Thermo Fisher Scientific nNOS Polyclonal Antibody
Thermo Fisher Scientific의 nNOS Polyclonal Antibody는 인간, 마우스, 랫드, 비인간 영장류에서 반응성을 가지며, Western blot 및 IHC(P) 실험에 적합합니다. 구아니아피그 유래 IgG 항체로, 합성 펩타이드 항원을 기반으로 제작되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Guinea pig / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human nNOS conjugated to blue carrier protein was used as the antigen |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
- The peptide is homologous in mouse and shares 96% identity with rat sequence.
- Specificity of this antibody: nNOS.
Target Information
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O₂) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme).
Several distinct NOS isoforms have been described and shown to represent the products of three distinct genes. These include two constitutive Ca²⁺/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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