Thermo Fisher Scientific gamma Tubulin Monoclonal Antibody (TU-30)
Thermo Fisher Scientific의 gamma Tubulin Monoclonal Antibody (TU-30)는 다양한 종에서 반응하며, 세포골격 및 MTOC 연구에 적합합니다. Western blot, ICC/IF 등 다중 응용 가능. 고순도 Protein A 정제, 액상 형태로 안정적이며 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL | View 8 publications |
| Flow Cytometry (Flow) | Assay-dependent | – |
| ELISA | – | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Bovine, Chicken, Human, Mouse, Plant, Pig, Protozoa, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TU-30 |
| Immunogen | C-terminal peptide of gamma-Tubulin conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1075282 |
Product Specific Information
This antibody recognizes the C-terminus (amino acids 434–449 in human) of gamma-tubulin, a 48 kDa structural constituent of the cytoskeleton and microtubule organizing center (MTOC).
The epitope is located in the amino acid sequence TRPDYI (aa439–444 in human), which is present on human gamma-tubulin 1 but not on gamma-tubulin 2.
Immunocytochemistry Staining Technique:
- Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; or
- Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C.
The antibody TU-30 stains only fixed cells.
Western Blot: Reducing conditions recommended.
Target Information
This gene encodes a protein that interacts with thyroid hormone receptors, which function as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain and may play a role in early-stage non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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