Thermo Fisher Scientific gamma Tubulin Monoclonal Antibody (TU-30)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody recognizes the C-terminus (amino acids 434–449 in human) of gamma-tubulin, a 48 kDa structural constituent of the cytoskeleton and microtubule organizing center (MTOC).
The epitope is located in the amino acid sequence TRPDYI (aa439–444 in human), which is present on human gamma-tubulin 1 but not on gamma-tubulin 2.

Immunocytochemistry Staining Technique:

1. Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; or
2. Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C.
   The antibody TU-30 stains only fixed cells.

Western Blot: Reducing conditions recommended.

Target Information

This gene encodes a protein that interacts with thyroid hormone receptors, which function as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain and may play a role in early-stage non-small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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