Thermo Fisher Scientific MUC1 Monoclonal Antibody (GP1.4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:25-1:200

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 4 publications 4 publications

Flow Cytometry (Flow)

1:25-1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GP1.4

Immunogen

Human milk fat globule. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MUC1,uniProtId:P15941-1,ncbiNodeId:9606,antigenRange:1-1255,antigenLength:1255,antigenImageFileName:MA1-06503_MUC1_P15941-1_House_mouse.svg,antigenImageFileNamePDP:MA1-06503_MUC1_P15941-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_558126

Product Specific Information

MA1-06503 detects episialin in human samples.

MA1-06503 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry, and immunohistochemistry.

The MA1-06503 immunogen is a human milk fat globule.

Store at 4ºC or in small aliquots at -20ºC if preferred. Avoid freeze-thaw cycles.

Target Information

MUC1 (Mucin 1, Episialin, MAM-6, CA 15-3, PEM and EMA) is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. MUC1 is a transmembrane glycoprotein with a large mucin-like extracellular domain that matures through several intermediate forms generated by proteolysis, and sequential addition and processing of numerous O-linked glycans that are heavily sialylated. MUC1 is highly polymorphic and each allele encodes a product that contains a different number of repeats (between 30 and 90) leading to large differences in molecular weight of the protein. MUC1 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells, and is expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. Overexpression, aberrant intracellular localization, and changes in glycosylation of the MUC1 protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of the MUC1 gene have been reported, but the full-length nature of only some has been determined. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.