Thermo Fisher Scientific Clathrin Heavy Chain Monoclonal Antibody (X22)
Clathrin Heavy Chain 단백질을 검출하는 Thermo Fisher Scientific의 단일클론 항체(X22). Western blot, IHC, ICC/IF, IP 등 다양한 응용 가능. 고순도 Protein A 정제, 비결합형 액상 포맷으로 제공. -20°C 보관, 연구용 전용.
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Thermo Fisher Scientific Clathrin Heavy Chain Monoclonal Antibody (X22)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:500 | 21 |
| Immunohistochemistry (IHC) | - | 10 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 67 |
| Flow Cytometry (Flow) | 1/200 | 1 |
| Immunoprecipitation (IP) | Assay-dependent | 5 |
| Neutralization (Neu) | - | 2 |
| Immunomicroscopy (IM) | Assay-dependent | 1 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Hamster, Human, Mouse, Non-human primate, Rat |
| Published Species | Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Pig, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | X22 |
| Immunogen | Purified human brain clathrin heavy chain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 6 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2083179 |
Available Conjugated Formats
Product Specific Information
MA1-065 detects clathrin heavy chain in non-human primate, bovine, human, rat, and mouse tissues as well as hamster (CHO) cells.
It has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures.
By Western blot, this antibody recognizes a single ~180 kDa protein representing the clathrin heavy chain from bovine brain extract.
Immunoprecipitation of triskelions with MA1-065 allows both the clathrin heavy chain and the associated light chain polypeptides to be examined.
Electron microscopy and proteolysis mapping demonstrate that MA1-065 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.
Target Information
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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