Thermo Fisher Scientific Clathrin Heavy Chain Monoclonal Antibody (X22)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 21 publications 21 publications

Immunohistochemistry (IHC)

-

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 67 publications 67 publications

Flow Cytometry (Flow)

1/200

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 5 publications 5 publications

Neutralization (Neu)

-

View 2 publications 2 publications

Immunomicroscopy (IM)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

X22

Immunogen

Purified human brain clathrin heavy chain. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Clathrin Heavy Chain,uniProtId:Q00610-1,ncbiNodeId:9606,antigenRange:1-1675,antigenLength:1675,antigenImageFileName:MA1-065_Clathrin_Heavy_Chain_Q00610-1_House_mouse.svg,antigenImageFileNamePDP:MA1-065_Clathrin_Heavy_Chain_Q00610-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 647
• Request custom conjugation

Form

Liquid

Concentration

6 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2083179

Product Specific Information

MA1-065 detects clathrin heavy chain in non-human primate, bovine, human, rat and mouse tissues as well as hamster (CHO) cells.

MA1-065 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody recognizes a single ~180 kDa protein representing the clathrin heavy chain from bovine brain extract. Immunoprecipitation of triskelions with MA1-065 allows both the clathrin heavy chain and the associated light chain polypeptides to be examined.

The MA1-065 immunogen is purified human brain clathrin heavy chain. Electron microscopy and proteolysis mapping demonstrate that MA1-065 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.

Target Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.