Thermo Fisher Scientific Ebola Virus VP40 (subtype Sudan, Zaire) Monoclonal Antibody (2614)

Applications

Western Blot (WB)

• Assay-dependent

ELISA

• 1:20–1:200

Product Specifications

Product Specific Information

• Specificity: Reactive with VP40 of Sudan & Zaire sequences; reactive with VLPs.
• Purified preparation consists of >90% pure mouse monoclonal antibody purified from ascites fluid by Protein A chromatography.
• Final formulation: 100 µg/mL in 0.01 M PBS, pH 7.2, containing 0.1% sodium azide.
• Each vial contains 1.0 mL.
• No stabilizing proteins added; store at 4°C until use.
• Working dilution to be determined by the user. Suggested starting ranges:
  • ICC/IF: 1:10–1:50
  • ELISA: 1:20–1:200

Target Information

Ebola virus is an enveloped, negative-stranded RNA virus belonging to the family Filoviridae. It exhibits a distinctive morphology similar to Marburg virus but lacks antigenic similarity. The genome is a nonsegmented RNA strand encoding seven structural proteins:

• NP (104 kDa) – Major nucleoprotein
• GP (125 kDa) – Glycoprotein (surface spikes, glycosylated)
• L protein (180 kDa)
• VP40 (40 kDa)
• ViPX (35 kDa)
• VP30 (30 kDa)
• VP24 (24 kDa)

All proteins are encoded by monocistronic mRNA transcripts complementary to virion RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.