Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), Functional Grade

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

View 5 publications 5 publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

View 2 publications 2 publications

Inhibition Assays (IA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Cat, Human, Non-human primate

Published species

Human, Non-human primate

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

W6/32

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• Unconjugated
• Biotin
• FITC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1076699

Product Specific Information

This antibody will not cross-react with rabbit.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Purity is >95% by SDS-PAGE.

Western blot should be performed under non-reducing conditions. It has also been successfully used in Mass Cytometry (CyTof).

Target Information

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.