Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), Functional Grade
HLA-A, B, C 항원을 인식하는 기능 등급 단일클론 항체로 다양한 면역분석(WB, IHC, Flow 등)에 사용 가능. 인간 및 비인간 영장류 반응성. 고순도(>95%), 낮은 엔도톡신 수준, 비보존제 PBS 용액 형태. 연구용으로만 사용.
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Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), Functional Grade
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 2 publications |
| Flow Cytometry (Flow) | Assay-dependent | 5 publications |
| ELISA | Assay-dependent | 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Functional Assay | Assay-dependent | 2 publications |
| Inhibition Assays (IA) | - | 1 publication |
| Miscellaneous (PubMed) | - | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Bovine, Cat, Human, Non-human primate |
| Published Species | Human, Non-human primate |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | W6/32 |
| Immunogen | Membrane of human tonsil cells |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1076699 |
Additional Formats
Product Specific Information
- This antibody does not cross-react with rabbit.
- Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
- Purity: >95% by SDS-PAGE.
- Western blot should be performed under non-reducing conditions.
- Successfully used in Mass Cytometry (CyTOF).
Target Information
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with beta2 microglobulin. They carry foreign determinants crucial for immune recognition by cytotoxic T cells, playing key roles in anti-viral and anti-tumor defense.
Human HLA-class I antigens include HLA-A, HLA-B, and HLA-C molecules.
HLA and MHC antibodies are essential in Immunopeptidomics for identifying and characterizing neoantigens through LC-MS/MS analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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