Thermo Fisher Scientific GDNF Polyclonal Antibody, PeproTech
Rabbit 유래 Polyclonal Anti-GDNF 항체로 인간 및 랫트 시료에 반응. Western blot과 ELISA에 적합하며 0.1–2.0 µg/mL 농도에서 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체로 신경영양 인자 연구에 활용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | 1 publication |
| In vitro Assay (IV) | - | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human GDNF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930779 |
Product Specific Information
AA Sequence of recombinant protein:
MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human GDNF. Anti-Human GDNF-specific antibody was purified by affinity chromatography employing an immobilized Human GDNF matrix.
Sandwich ELISA:
To detect Human GDNF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GDNF (500-P81Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human GDNF.
Western Blot:
To detect Human GDNF by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human GDNF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was later shown to rescue these neurons in animal models of Parkinson’s disease. GDNF is approximately 100 times more efficient as a survival factor for spinal motor neurons than neurotrophins. It enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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