Thermo Fisher Scientific GDNF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human GDNF. Anti-Human GDNF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hGDNF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GDNF (500-P81) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hGDNF.

Western Blot:
To detect hGDNF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 mg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hGDNF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P81BT-1MG will be provided as 2 × 500 µg.

Target Information

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and shown to rescue these neurons in animal models of Parkinson’s disease. GDNF is about 100 times more efficient as a survival factor for spinal motor neurons than the neurotrophins. It enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.