Thermo Fisher Scientific Histone H3 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Histone H3. Validated for WB, IHC, ICC/IF, and ChIP applications. High specificity without cross-reactivity to other histones. Supplied as liquid form at 33 µg/mL, stored at -20°C for research use only.
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- PA517697
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- pk
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Thermo Fisher Scientific Histone H3 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:250 |
| ChIP assay (ChIP) | 1.5 µg / 1×10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the carboxy-terminal sequence of human histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 33 µg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10980629 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other histones.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of histone H3, such as methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression. The N-terminal tail of histone H3 undergoes these modifications to regulate cellular processes.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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