Thermo Fisher Scientific N-Ras Recombinant Rabbit Monoclonal Antibody (10H6L11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

10H6L11

Immunogen

Peptides corresponding to human N-Ras [1) aa83-aa98 2) aa166-aa180] if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:N-Ras,uniProtId:P01111-1,ncbiNodeId:9606,antigenRange:83-98|166-180,antigenLength:189,antigenImageFileName:703435_N-Ras_P01111-1_Rabbit.svg,antigenImageFileNamePDP:703435_N-Ras_P01111-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2784586

Product Specific Information

This antibody is against aa83-98 (AINNSKSFADINLYRE) as confirmed in ELISA testing.

This antibody is predicted to react with Pig, Rat, Rabbit.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

NRAS (GTPase NRas) is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML) and Noonan syndrome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.