Thermo Fisher Scientific CISD2 Monoclonal Antibody (OTI4F8), TrueMAB
CISD2 단백질을 표적으로 하는 단일클론 항체로, Western blot 및 IHC(Paraffin)에 적합. 인간, 마우스, 랫트 반응성. E.coli에서 생산된 full-length recombinant protein을 면역원으로 사용. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4F8 |
| Immunogen | Full length human recombinant protein of human CISD2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Defects in the CISD2 (CDGSH iron sulfur domain 2) gene cause the neurodegenerative disorder Wolfram syndrome 2.
CISD2 is a zinc finger protein localized to the endoplasmic reticulum and mitochondria, binding an iron/sulfur cluster.
It interacts with Bcl-2 and can be displaced by the BH3-only protein BIK, contributing to regulation of BIK-initiated autophagy.
CISD2 deficiency in mice leads to mitochondrial breakdown, autophagic cell death, and premature aging phenotypes.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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