Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI6A10), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

• For reconstitution, add 100 µL distilled water to obtain a final concentration of ~1 mg/mL.
• For conjugation experiments, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and participates in DNA excision and mismatch repair pathways. It binds to CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferative cells and tumor prognosis, being expressed during early G1 and S phases.
Located in the nucleus, PCNA acts as a cofactor for DNA polymerase delta and forms a homotrimer to enhance leading strand synthesis.
Upon DNA damage, PCNA undergoes ubiquitination and functions in the RAD6-dependent repair pathway. Two transcript variants encode the same protein, with pseudogenes found on chromosome 4 and the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.