Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI4E3), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and involved in DNA excision and mismatch repair pathways.
It binds to CDK inhibitor p21, structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells and helps assess tumor proliferation status.
Located in the nucleus, PCNA acts as a cofactor for DNA polymerase delta, forming a homotrimer that enhances leading strand synthesis.
Upon DNA damage, PCNA is ubiquitinated and participates in the RAD6-dependent DNA repair pathway.
Two transcript variants encode the same protein; pseudogenes are found on chromosome 4 and the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.