Thermo Fisher Scientific PSMB9 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mixture of synthetic peptides corresponding to residues C R(207) V I L G N/D E L P K F Y D E(220) of human and mouse proteasome 20S LMP2. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PSMB9,uniProtId:P28076-1,ncbiNodeId:10090,antigenRange:207-219,antigenLength:219,antigenImageFileName:PA1-1960_PSMB9_P28076-1_Rabbit.svg,antigenImageFileNamePDP:PA1-1960_PSMB9_P28076-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2284519

Product Specific Information

PA1-1960 detects proteasome 20S LMP2 from human and mouse cells.

PA1-1960 can be successfully used in Western blot procedures. By Western blot this antibody detects an ~23 kDa protein representing proteasome 20S LMP2 from mouse embryo fibroblast cell extract. This antibody also recognizes a band at ~24 kDa, which may correspond to LMP2 precursor protein.

The PA1-1960 immunogen is a mixture of synthetic peptides corresponding to residues C R(207) V I L G N/D E L P K F Y D E(220) of human and mouse proteasome 20S LMP2. These peptides are available for use in neutralization and control procedures.

PA1-1960 can be used with blocking peptide PEP-176.

Target Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC in the immunoproteasome. Proteolytic processing is required to generate a mature subunit.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.