Thermo Fisher Scientific LDHA Recombinant Rabbit Monoclonal Antibody (4H18L6), Alexa Fluor Plus 555

Applications

Tested Dilution

Publications

Western Blot (WB)

1:400-1:800

-

Immunocytochemistry (ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

4H18L6

Immunogen

Peptides corresponding to human LDHA [1) aa211-aa229 2) aa2-aa19] if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LDHA,uniProtId:P00338-1,ncbiNodeId:9606,antigenRange:2-19|211-229,antigenLength:332,antigenImageFileName:702747RP555_LDHA_P00338-1_Rabbit.svg,antigenImageFileNamePDP:702747RP555_LDHA_P00338-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555

View additional formats

• Unconjugated
• Alexa Fluor Plus 488
• Alexa Fluor Plus 647
• Request custom conjugation

Excitation/Emission Max

558/572 nm View spectra

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

proprietary buffer, pH 6.8

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Applications Tested: This 702747RP555 antibody (4H18L6) has been tested by western blot and immunocytochemistry analysis of PC-3 cells. This may be used for immunocytochemistry at 5 µg/ml and for Western blotting at less than or equal to 1.25 µg per test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.