Thermo Fisher Scientific LDHA Recombinant Rabbit Monoclonal Antibody (4H18L6), Alexa Fluor Plus 647

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Request custom conjugation

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies utilize proprietary dye chemistry to produce brighter and more stable fluorescent signals compared to traditional Alexa Fluor™ conjugates. These antibodies ensure improved signal-to-noise ratio, superior specificity, and consistent performance due to recombinant production methods.

Usage Note:
Centrifuge the conjugate solution briefly before use and apply only the supernatant to minimize nonspecific background caused by aggregates.

Applications Tested:
This antibody (4H18L6, Cat. No. 702747RP647) has been validated for:

• Western blot analysis of PC-3 cells (≤1.25 µg/mL)
• Immunocytochemistry (5 µg/mL)

Excitation: 658 nm
Emission: 675 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and lactate in glycolysis, involving NADH/NAD⁺ cycling.
In mammals, LDH is encoded by three genes:

• LDHA (M form) – muscle type, hypoxia-inducible, regulated by HIF1α
• LDHB (H form) – heart type, upregulated in many cancers
• LDHC (X form) – testis type

Human LDHs share 84–89% sequence similarity and 69–75% amino acid identity. Mutations in LDHA can cause Glycogen Storage Disease 11 (GSD11).

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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