Thermo Fisher Scientific LDHA Recombinant Rabbit Monoclonal Antibody (4H18L6), Alexa Fluor Plus 647
LDHA 단백질을 표적으로 하는 Recombinant Rabbit Monoclonal Antibody로, Alexa Fluor Plus 647 형광 표지. Western blot 및 Immunocytochemistry에 최적화. 높은 특이성과 일관된 로트 간 재현성 제공. 4°C 보관, 동결 금지.
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- 702747RP647x (2개 옵션)
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400–1:800 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4H18L6 |
| Immunogen | Peptides corresponding to human LDHA (aa211–aa229, aa2–aa19) |
| Conjugate | Alexa Fluor™ Plus 647 |
| Excitation / Emission Max | 658 / 675 nm |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A |
| Storage Buffer | Proprietary buffer, pH 6.8 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
Additional Formats
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies utilize proprietary dye chemistry to produce brighter and more stable fluorescent signals compared to traditional Alexa Fluor™ conjugates. These antibodies ensure improved signal-to-noise ratio, superior specificity, and consistent performance due to recombinant production methods.
Usage Note:
Centrifuge the conjugate solution briefly before use and apply only the supernatant to minimize nonspecific background caused by aggregates.
Applications Tested:
This antibody (4H18L6, Cat. No. 702747RP647) has been validated for:
- Western blot analysis of PC-3 cells (≤1.25 µg/mL)
- Immunocytochemistry (5 µg/mL)
Excitation: 658 nm
Emission: 675 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and lactate in glycolysis, involving NADH/NAD⁺ cycling.
In mammals, LDH is encoded by three genes:
- LDHA (M form) – muscle type, hypoxia-inducible, regulated by HIF1α
- LDHB (H form) – heart type, upregulated in many cancers
- LDHC (X form) – testis type
Human LDHs share 84–89% sequence similarity and 69–75% amino acid identity. Mutations in LDHA can cause Glycogen Storage Disease 11 (GSD11).
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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