Thermo Fisher Scientific IDO Chimeric Recombinant Mouse Monoclonal Antibody (7H8L17)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:200

-

Flow Cytometry (Flow)

0.0625-1 µg/test

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG

Expression System

ExpiCHO

Class

Recombinant Monoclonal

Type

Antibody

Clone

7H8L17

Immunogen

Protein corresponding to human IDO (aa1-aa403) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IDO,uniProtId:P14902-1,ncbiNodeId:9606,antigenRange:1-403,antigenLength:403,antigenImageFileName:702743M_IDO_P14902-1_House_mouse.svg,antigenImageFileNamePDP:702743M_IDO_P14902-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

This antibody has Rabbit Variable heavy and light chains, and Mouse constant domains. It recognizes human IDO protein. This chimeric antibody is recognized by anti-mouse secondary antibodies and has no cross reactivity to anti-rabbit secondary antibodies.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin. By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction. The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.