Thermo Fisher Scientific IDO Chimeric Recombinant Mouse Monoclonal Antibody (7H8L17)
Human IDO 단백질을 인식하는 Thermo Fisher의 키메릭 재조합 마우스 단클론 항체로, WB, ICC/IF, Flow Cytometry에 적합. ExpiCHO 시스템에서 발현된 비결합형 항체이며, Protein A/G로 정제됨. 연구용으로만 사용.
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Thermo Fisher Scientific IDO Chimeric Recombinant Mouse Monoclonal Antibody (7H8L17)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 0.0625–1 µg/test |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG |
| Expression System | ExpiCHO |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7H8L17 |
| Immunogen | Protein corresponding to human IDO (aa1–aa403) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
This antibody contains rabbit variable heavy and light chains with mouse constant domains. It specifically recognizes human IDO protein.
This chimeric antibody is detected by anti-mouse secondary antibodies and does not cross-react with anti-rabbit secondary antibodies.
It is recommended to titrate the antibody carefully for optimal performance in the desired assay.
Target Information
IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of regulatory molecules such as tryptophan, serotonin, and melatonin. This process initiates the formation of biologically active kynurenines.
IDO1 is widely expressed in human tissues, macrophages, and dendritic cells. During inflammation, interferons (IFNs) induce IDO1 expression, contributing to antimicrobial defense by degrading tryptophan, thereby inhibiting pathogen growth.
IDO1 also plays an essential role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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