Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), PE, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496, 661, 737
• Brilliant Violet 421
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Violet, Yellow series
• PE-Cyanine5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and by most peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis, depending on assay conditions. It is used as a phenotypic marker for TCR beta-expressing T cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse thymocytes and splenocytes. Recommended ≤ 0.5 µg per test (100 µL final volume). Optimal titration is recommended per assay.

Optical Properties:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

T cell receptors (TCR) enable discrimination between foreign and self-peptides presented by MHC class II molecules, essential for adaptive immunity. Recognition of self-peptides is linked to autoimmune diseases, and mutant self-peptides are associated with tumors. TCR engagement by bacterial superantigens can cause toxic shock syndrome. Autoantibodies to V beta segments of TCRs have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), potentially modulating inflammatory autoimmune responses.

Human T cells typically express TCR alpha-beta with either CD4 or CD8 (single positive). A smaller subset lacks both (double negative), which can increase in autoimmune or immunodeficiency conditions. Gamma-delta T cells, primarily epithelial, show less diversity and distinct antigen recognition, with some subsets exhibiting antitumor and immunoregulatory activity.

For Research Use Only. Not for diagnostic use or resale without authorization.

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