Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), PE, eBioscience
Mouse TCR beta 사슬을 인식하는 H57-597 단클론 항체로, PE 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 0.5 µg/test로 사용 가능합니다. 488–561 nm 여기, 578 nm 방출 특성을 가지며, T세포 분석 및 표지에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 1 |
| Flow Cytometry (Flow) | 0.5 µg/test | 69 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H57-597 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466065 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 496, 661, 737
- Brilliant Violet 421
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue, Violet, Yellow series
- PE-Cyanine5, PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and by most peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis, depending on assay conditions. It is used as a phenotypic marker for TCR beta-expressing T cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse thymocytes and splenocytes. Recommended ≤ 0.5 µg per test (100 µL final volume). Optimal titration is recommended per assay.
Optical Properties:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
T cell receptors (TCR) enable discrimination between foreign and self-peptides presented by MHC class II molecules, essential for adaptive immunity. Recognition of self-peptides is linked to autoimmune diseases, and mutant self-peptides are associated with tumors. TCR engagement by bacterial superantigens can cause toxic shock syndrome. Autoantibodies to V beta segments of TCRs have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), potentially modulating inflammatory autoimmune responses.
Human T cells typically express TCR alpha-beta with either CD4 or CD8 (single positive). A smaller subset lacks both (double negative), which can increase in autoimmune or immunodeficiency conditions. Gamma-delta T cells, primarily epithelial, show less diversity and distinct antigen recognition, with some subsets exhibiting antitumor and immunoregulatory activity.
For Research Use Only. Not for diagnostic use or resale without authorization.
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