Thermo Fisher Scientific Phospho-SMAD2 (Ser255) Recombinant Rabbit Monoclonal Antibody (JF0882)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select optimal candidates for production.
Advantages include:

• Improved specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to the larger rabbit immune repertoire

Target Information

SMAD2 (MADH2, MAD2) regulates cellular processes such as proliferation, apoptosis, and differentiation. It interacts with TGF-beta receptors via the SMAD anchor for receptor activation, a key event in TGF-beta signaling.
Phosphorylation of threonine 8 by ERK1 enhances SMAD2 transcriptional activity, negatively regulated by calmodulin. Upon TGF-beta signaling, SMAD2 is phosphorylated, dissociates from SARA, and associates with SMAD4. This complex translocates to the nucleus to regulate transcription. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, mediating activin signaling.
Alternatively spliced transcript variants encoding SMAD2 have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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