
Thermo Fisher Scientific Rat IgG1 kappa Isotype Control (eBRG1), eFluor 450, eBioscience
Flow cytometry용 Rat IgG1 kappa 동종 대조 항체로, eFluor 450 형광체 결합. 실험 항체와 동일 농도로 사용 권장. 405 nm에서 여기, 445 nm에서 방출. 음성 대조 및 형광 분석에 적합.
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Applications
- Flow Cytometry (Flow): Assay-Dependent
- Control (Ctrl): Assay-Dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBRG1 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1271988 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 506, 570, 615, 660
- FITC
- Functional Grade
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
The monoclonal rat IgG1, kappa antibody is useful as an isotype control immunoglobulin.
- Applications Reported: Suitable for flow cytometric analysis.
- Applications Tested: Tested on mouse splenocytes; use at the same concentration as experimental antibody.
- Fluorochrome: eFluor® 450 (alternative to Pacific Blue®).
- Excitation/Emission: 405 nm / 445 nm (Violet laser).
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies commonly used in research and diagnostics. These antibodies are produced by rats in response to antigens and can be purified from serum or hybridoma culture supernatants.
Rat IgG1 is often used as a negative control because it does not bind to most mammalian antigens. It is applicable in immunohistochemistry, flow cytometry, and western blotting for antigen detection.
Gene targeting with rat models enables investigation of gene functions and disease mechanisms through knockout or gene modification studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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