Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), eFluor 450, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

Assay-Dependent

Control (Ctrl)

Assay-Dependent

Product Specifications

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Isotype Control

Clone

eBR2a

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

View additional formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 506
• eFluor 570
• eFluor 615
• eFluor 660
• FITC
• Functional Grade
• Pacific Orange
• PE
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1272002

Product Specific Information

Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.

Applications Reported: Rat IgG2a Isotype Control eFluor® 450 has been reported for use in flow cytometric analysis. .

Applications Tested: This Rat IgG2a Isotype Control has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.