Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Secondary Antibody, Biotin
Thermo Fisher의 Donkey anti-Rat IgG (H+L) Biotin 결합 2차 항체로, ELISA, Western blot, ICC/IF, Flow cytometry에 사용 가능. 높은 특이성과 감도로 Rat IgG 검출에 적합하며, 친화 크로마토그래피로 정제되어 신뢰성 높은 결과 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:50,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535520 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful in detection, sorting, or purification of specific targets. Secondary antibodies offer enhanced versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Typically, these antibodies are produced by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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