Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Secondary Antibody, FITC
Thermo Fisher Scientific의 Donkey anti-Rat IgG (H+L) FITC 결합 2차 항체로, Rat IgG 검출에 적합합니다. ICC/IF 및 유세포분석용으로 사용되며, 높은 특이성과 감도를 제공합니다. FITC 형광(498/517 nm)으로 시각화 가능하며, 안정적인 액상 형태로 제공됩니다.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 2.5 µg/mL |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host/Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C (short term); -20°C (long term). Avoid freeze/thaw cycles. Store in the dark. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535517 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats
- AP
- Biotin
- HRP
- TRITC
- Custom conjugation available upon request
Product Specific Information
Each antibody lot is tested for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP).
Target Information
Anti-Rat secondary antibodies are affinity-purified reagents with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of rat IgG. Secondary antibodies enhance versatility and sensitivity by allowing multiple detection systems such as HRP, AP, or fluorescence. These antibodies are typically produced by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to ensure high specificity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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