Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Secondary Antibody, AP
Thermo Fisher Scientific의 Donkey anti-Rat IgG (H+L) AP 결합 2차 항체로, Rat IgG 검출에 사용됩니다. Western blot, IHC, ELISA 등 다양한 응용에 적합하며, 높은 민감도와 특이성을 제공합니다. 친화 크로마토그래피로 정제되어 신뢰성 높은 결과를 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535519 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Biotin
- FITC
- HRP
- TRITC
- Request custom conjugation
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Target Information
Anti-Rat secondary antibodies are affinity-purified with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of rat IgG targets.
Secondary antibodies provide versatility across detection systems (HRP, AP, fluorescence) and enhance sensitivity via signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
They are typically produced by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to ensure high specificity and performance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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