Thermo Fisher Scientific Chicken anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin

Applications

Tested Dilution

Western Blot (WB)

1:10,000-1:50,000

Immunocytochemistry (ICC/IF)

1:500-1:20,000

Flow Cytometry (Flow)

1:500-1:20,000

ELISA (ELISA)

1:500-1:20,000

Product Specifications

Species Reactivity

Rat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Biotin Biotin Biotin

View additional formats

• [Alexa Fluor 488 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Chicken/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Chicken/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Chicken/)
• [AP](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Chicken/)
• [HRP](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Chicken/)
• Request custom conjugation

Form

Liquid

Concentration

2.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2535514

Target

IgG

Cross Adsorption

Against human and rabbit IgG and serum proteins

Antibody Form

Whole Antibody

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.