Thermo Fisher Scientific Granzyme B Monoclonal Antibody (NGZB), PE-eFluor 610, eBioscience

Thermo Fisher Scientific Granzyme B Monoclonal Antibody (NGZB), PE-eFluor 610, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 0.25 µg/test
• Publications: View 36 publications

Product Specifications

Available Formats

• APC
• APC-eFluor 780
• eFluor 450
• eFluor 660
• FITC
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This NGZB monoclonal antibody reacts with mouse Granzyme B, a member of the granzyme serine protease family located in the granules of cytotoxic T cells and NK cells. Granzyme B plays a key role in apoptosis induction via mannose 6-phosphate receptor-mediated pathways.

• Applications Reported: Intracellular staining followed by flow cytometric analysis
• Applications Tested: Tested with stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00)
• Recommended Protocol: BestProtocols® - Protocol A
• Usage: ≤ 0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Excitation/Emission: 488–561 nm / 607 nm; suitable for Blue, Green, and Yellow-Green lasers
• Filter Recommendation: 610/20 band pass filter (PE-Texas Red® equivalent)
• Light Sensitivity: Protect from light to prevent photo-induced oxidation
• Fixation: Compatible with IC Fixation Buffer (Product # 00-8222) or Fix/Lyse Solution (Product # 00-5333); store up to 3 days at 4°C in dark
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Granzyme B is a serine protease found in cytotoxic T lymphocytes (CTLs) and NK cells. It induces apoptosis through caspase activation and mitochondrial pathways. Granzyme B interacts with mannose 6-phosphate receptor as a death receptor during cytotoxic T cell-induced apoptosis. It cleaves caspase-3 and Bid protein to trigger DNA fragmentation and cell death. Various granzymes (A–G) have been identified in mouse CTLs and NK cells.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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