Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), PE-eFluor 610, eBioscience

Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), PE-eFluor 610, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular Staining)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 70 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 661 / 737 / 805
• Brilliant Violet 650
• eFluor 450 / 506 / 660
• FITC
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The 4S.B3 monoclonal antibody reacts with human interferon-gamma (IFN-γ), a 17 kDa cytokine produced by activated T and NK cells with antiviral and antiparasitic activity. It synergizes with TNF-α to inhibit proliferation of normal and transformed cells and exhibits immunomodulatory effects on various cell types expressing IFN-γ receptors.

Applications Reported:
Intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-titrated and validated using intracellular staining and flow cytometry of stimulated human peripheral blood cells with the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00) following BestProtocols® (Protocol A).
Recommended use: 5 µL (0.5 µg) per test in a final volume of 100 µL (10⁵–10⁸ cells/test).

Fluorophore Information:
PE-eFluor® 610 is excited by 488–561 nm lasers and emits at 607 nm. Use a 610/20 band pass filter (PE-Texas Red® equivalent). Protect from light due to photo-induced oxidation sensitivity.

Fixation:
Samples may be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal signal loss.

Laser Compatibility:

• Excitation: 488–561 nm
• Emission: 607 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Interferon gamma (IFN-γ, Type II interferon) is a macrophage activation factor produced mainly by T-lymphocytes and NK cells in response to antigens and cytokines. It functions as a homodimer (~45 kDa) with antiviral, antiproliferative, and immunomodulatory activities, including induction of MHC I/II, macrophage activation, and enhanced antibody secretion by B cells. Human IFN-γ is species-specific and does not cross-react with mouse IFN-γ.
Mutations in the IFN-γ gene are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures or resale.

제품 이미지