Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), PE-eFluor 610, eBioscience
Mouse 및 Rat IL-17A를 특이적으로 인식하는 eBio17B7 단클론 항체로, PE-eFluor 610 형광체가 결합되어 Flow Cytometry에 최적화됨. 염증성 사이토카인 연구 및 Th17 세포 분석에 적합하며, 4°C 암조건 보관 권장.
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Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), PE-eFluor 610, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 82 publications |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio17B7 |
| Conjugate | PE-eFluor™ 610 |
| Excitation / Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574655 |
Additional Conjugate Formats
- Alexa Fluor 488
- APC
- Biotin
- Brilliant UV 395 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 650 / 711 / 786
- eFluor 450 / 506 / 660
- FITC
- PE
- PE-Cyanine7
- PerCP-Cyanine5.5
- Custom conjugation available
Product Specific Information
The eBio17B7 antibody reacts with mouse and rat IL-17A without cross-reactivity to IL-17F.
IL-17A is a CD4+ T cell-derived cytokine promoting inflammatory responses and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
- Applications Reported: Intracellular staining followed by flow cytometric analysis.
- Applications Tested: Tested by intracellular staining and flow cytometric analysis of Th17-polarized mouse splenocytes using Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00).
- Recommended ≤ 0.25 µg/test (100 µL final volume).
- Optimal titration recommended.
- Staining Compatibility: Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00).
- Fluorophore: PE-eFluor® 610 (Excitation: 488–561 nm, Emission: 607 nm).
- Recommended filter: 610/20 band pass (PE-Texas Red® equivalent).
- Sensitive to photo-induced oxidation; protect from light.
- Fixation: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54).
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory diseases.
IL-17A forms a disulfide-linked homodimer (32 kDa, 136 amino acids) and signals through the IL-17 receptor (IL-17R/CDw217).
It enhances expression of ICAM-1 and stimulates epithelial, endothelial, and fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2.
IL-17A regulates NF-κB and MAPK pathways, induces COX-2 expression, and promotes nitric oxide production.
For Research Use Only. Not for use in diagnostic procedures.
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