Thermo Fisher Scientific beta Catenin Polyclonal Antibody (CAT-15)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 46 publications 46 publications

Immunohistochemistry (IHC)

-

View 20 publications 20 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 36 publications 36 publications

ELISA (ELISA)

0.1-1 mg/mL

-

Immunoprecipitation (IP)

5-10 µg

View 4 publications 4 publications

ChIP assay (ChIP)

-

View 9 publications 9 publications

Miscellaneous PubMed (Misc)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat, Xenopus

Published species

Amphibian, Avian, Dog, Human, Mouse, Pig, Rabbit, Rat, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Clone

CAT-15

Immunogen

A 15-amino acid synthetic peptide derived from the C-terminus of the human/mouse beta-catenin protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta Catenin,uniProtId:Q02248-1,ncbiNodeId:10090,antigenRange:781,antigenLength:781,antigenImageFileName:71-2700_beta_Catenin_Q02248-1_Rabbit.svg,antigenImageFileNamePDP:71-2700_beta_Catenin_Q02248-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533982

Product Specific Information

This antibody can be used to specifically immunoprecipitate the ~ 92 kDa beta-catenin protein from native cell lysates. It is suitable for use in immunoprecipitation (IP) and IP/western applications. Note that when the antibody is used for straight western blotting, cross-reactivity with a ~100 kDa protein of unknown identity is sometimes observed. Suggested positive control lysates include HeLa and A431.

Target Information

Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.