Thermo Fisher Scientific C-Jun Monoclonal Antibody (OTI9F5), TrueMAB
c-Jun 단백질을 인식하는 Mouse Monoclonal Antibody로 Western Blot 및 IHC(Paraffin)에 적합. E.coli에서 발현된 full-length 인간 JUN 단백질을 면역원으로 제작. 동결건조 형태로 제공되며, 재구성 시 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9F5 |
| Immunogen | Full length human recombinant protein of human JUN produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
It promotes activity of NR5A1 when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon cAMP pathway stimulation.
It is also involved in KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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