Thermo Fisher Scientific Growth Hormone Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified human growth hormone.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

<0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2549917

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is anterior pituitary tissue.

Target Information

Human growth hormone (hGH) is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The GH1 gene, along with 4 additional related genes, is located at the growth hormone locus on chromosome 17. The five genes share a remarkably high degree of sequence identity. Alternative splicing results in 5 isoforms ranging from approximately 19 kDa to 24 kDa. HGH is produced by the somatotroph cells in the pituitary. It acts through binding to membrane receptors that belong to the cytokine receptor subfamily. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Mutations or deletions in the gene lead to growth hormone deficiency and short stature.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.