Invitrogen™

Dynabeads™ Untouched™ Human T Cells Kit

Use this kit to isolate pure and viable untouched T-cells from PBMC by negative isolation. The kit depletes B cells,자세히 알아보기

Use this kit to isolate pure and viable untouched T-cells from PBMC by negative isolation. The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free T-cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched T cells
• Easy to use and to scale up
• No columns required

Methodology:
An antibody mix towards the non-T cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human T-cells can be directly analyzed in a flow cytometer and used in any application.

Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended

Starting Sample:
PBMC

사양

세포 유형T Cells (Whole Population)

제품 유형Cell Isolation Kit

최종 제품 유형Cells

분리 기술Negative Isolation

셀 수Processes ∼1 x 109 cells total

테스트 수100 Isolations

출력 생존력>95%

제품라인DYNAL™, Dynabeads™, Untouched™

수량1 kit

샘플 종류PBMC

배송 조건Room Temperature

시작 물질 셀 수1 x 107 PBMCs per isolation

타겟 종Human

Unit SizeEach