Thermo Fisher Scientific CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio927), FITC, eBioscience

Thermo Fisher Scientific CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio927), FITC, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 10 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• APC
• Biotin
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse.
Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naive mouse, representing a minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation triggering an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8.

The eBio927 monoclonal antibody has functional activity. The epitope recognized by eBio927 is distinct from eBio129c, allowing costaining, purification, and identification of pDCs.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of SJL splenocytes. Recommended ≤0.5 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Careful titration is recommended for optimal performance.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

BST2 (CD317, HM1.24 antigen, DAMP-2, tetherin) is an integral membrane protein involved in B-cell development and growth. It facilitates stromal cell-dependent growth of pre-B-cell lines and is highly expressed during B-cell development, as well as in T-cells, monocytes, NK cells, DCs, and myeloma cells.

BST2 also functions as an inhibitor of retrovirus release, antagonized by HIV-1 protein Vpu. Its depletion abolishes the viral requirement for Vpu, suggesting therapeutic potential for HIV/AIDS. BST2 dysfunction is also associated with stomatitis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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