Thermo Fisher Scientific VIM Monoclonal Antibody (OTI5C10), TrueMAB
인간 Vimentin 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC(P), ICC/IF에 적합합니다. 동결건조 형태로 제공되며, 1 mg/mL 농도 및 PBS 버퍼에 보존됩니다. 연구용으로 세포 구조 및 단백질 수송 연구에 활용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:150 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C10 |
| Immunogen | Full length human recombinant protein of human VIM produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Vimentin is a developmentally regulated intermediate filament protein found in cells of mesenchymal origin. It plays a role in intracellular transport of proteins between the nucleus and plasma membrane. Vimentin is expressed during early cellular development and later replaced by tissue-specific intermediate filament proteins.
It is involved in steroid synthesis regulation by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation by protein kinase leads to filament breakdown and cytoskeletal changes facilitating cholesterol transport to mitochondria, enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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