Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI11F12), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (Proliferating Cell Nuclear Antigen) is essential for DNA replication and participates in DNA excision and mismatch repair pathways.
It interacts with CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells and is associated with the G1 and S phases of the cell cycle.
Located in the nucleus, PCNA acts as a cofactor of DNA polymerase delta, forming a homotrimer that enhances DNA synthesis efficiency.
Upon DNA damage, PCNA becomes ubiquitinated and functions in the RAD6-dependent DNA repair pathway.
Two transcript variants encoding the same protein have been identified, along with pseudogenes on chromosome 4 and the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.