Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI13G4), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (Proliferating Cell Nuclear Antigen) is an essential cofactor for DNA polymerase delta, playing a critical role in DNA replication, excision repair, and mismatch repair pathways.
It interacts with CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker of proliferative cells and is useful for assessing tumor proliferation status.
It is primarily localized in the nucleus, functions as a homotrimer, and enhances leading-strand synthesis during replication.
Upon DNA damage, PCNA becomes ubiquitinated and participates in the RAD6-dependent DNA repair pathway.
Two transcript variants encoding the same protein have been identified, and pseudogenes exist on chromosome 4 and the X chromosome.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.