Thermo Fisher Scientific Phospho-Raf-1 (Ser259) Polyclonal Antibody
Raf-1 단백질의 Ser259 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC, IP 등 다양한 응용에 적합. Human 및 Mouse 시료 반응성. 항원 친화 크로마토그래피로 정제된 고품질 항체. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide RST-[S]-TPN corresponding to the phosphorylation site Ser259 of human Raf-1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: HeLa cells
- Cellular location: Cytoplasm, Cell membrane
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) that functions downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates the dual specificity protein kinases MEK1 and MEK2, which in turn activate the serine/threonine kinases ERK1 and ERK2. Activated ERKs regulate cell physiology, gene expression, cell cycle, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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