Thermo Fisher Scientific OCT4 Monoclonal Antibody (9B7), HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:1,000

-

Product Specifications

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

9B7

Immunogen

Full length human recombinant protein of human POU5F1 produced in E.coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:OCT4,uniProtId:Q01860-1,ncbiNodeId:9606,antigenRange:1-360,antigenLength:360,antigenImageFileName:MA1-104-HRP_OCT4_Q01860-1_House_mouse.svg,antigenImageFileNamePDP:MA1-104-HRP_OCT4_Q01860-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

HRP HRP HRP

View additional formats

• Unconjugated
• DyLight 488
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536810

Product Specific Information

MA1-104-HRP detects Oct4/Pou5F1 in human embryonic carcinoma cells.

By Western blot, this antibody detects a band at 40 kDa in human embryonal carcinoma cells, but not in negative control cells.

Target Information

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.