Thermo Fisher Scientific NCK2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:100

-

ELISA (ELISA)

1:2,000-1:5,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide peptide corresponding to a region derived from 94-107 amino acids of human NCK adaptor protein 2 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NCK2,uniProtId:O43639-1,ncbiNodeId:9606,antigenRange:94-107,antigenLength:380,antigenImageFileName:PA5-49726_NCK2_O43639-1_Rabbit.svg,antigenImageFileNamePDP:PA5-49726_NCK2_O43639-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 40% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2635179

Product Specific Information

The antibody detects endogenous level of total NCK2 protein.

Target Information

The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 and NCK2, which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.