
Thermo Fisher Scientific SPAG4 Polyclonal Antibody
SPAG4 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, ELISA에 적합하며, 인간 및 생쥐 시료에 반응. 항원 친화 크로마토그래피로 정제된 액상 항체로, PBS/glycerol 완충액에 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:30–1:150 |
| ELISA | 1:2,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 422–436 of Human sperm associated antigen 4 (SPAG4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.7 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2635183 |
Product Specific Information
This antibody detects endogenous levels of total SPAG4 protein.
Target Information
The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece, and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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