Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright 436, eBioscience
Armenian hamster 유래 IgG 동형 대조 항체로, Flow Cytometry용 Super Bright 436 형광 접합체입니다. 실험 항체의 비특이적 결합 평가에 적합하며, 405 nm 레이저로 여기되어 436 nm에서 방출됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBio299Arm |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717007 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 615 / 660
- FITC
- Functional Grade
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 600 / 645 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Use at the same concentration as the experimental antibody.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. A 450/50 bandpass filter or equivalent is recommended. Ensure the instrument can detect this fluorochrome.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Isotype control antibodies are used to distinguish non-specific background signal from specific antibody signal. They have no relevant specificity to a target antigen and serve as negative controls.
They are primarily used in flow cytometry but can also be applied in ChIP, immunohistochemistry, and gel shift assays.
Isotype controls should match the species and isotype of the primary antibody and, if directly labeled, use the same fluorochrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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