Thermo Fisher Scientific Phospho-ELK1 (Ser389) Polyclonal Antibody
Phospho-ELK1 (Ser389) 폴리클로날 항체로, MAP kinase 경로 관련 ELK1 단백질의 인산화 상태를 검출. Western blot, IHC, IP 등 다양한 응용 가능. 고순도(>95%) 및 1 mg/mL 농도, 연구용으로 적합.
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- PA536641
- 판매단위
- pk
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Thermo Fisher Scientific Phospho-ELK1 (Ser389) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Elk-1 around the phosphorylation site of Serine 389 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C for short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553629 |
Product Specific Information
- Detects endogenous protein at a molecular weight of approximately 45 kDa.
- Purity >95% by SDS-PAGE.
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors.
ELK1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1.
ELK1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that ELK1 is a good substrate for MAP kinase, the kinetics of ELK1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block ELK1 activation in vivo.
ELK1 is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for ELK1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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