Thermo Fisher Scientific H3K27me2 Monoclonal Antibody
H3K27me2 단일클론 항체로 Western blot, ICC/IF, ChIP 등 다양한 응용에 적합. 인간 히스톤 H3의 Lys27 이중메틸화 부위를 인식. 고순도 Mouse IgG 형식, 액상 형태로 제공되며 -20°C 보관 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | 1.5 µg |
| Peptide Array (Array) | 1:300 |
Product Specifications
| Specification | Detail |
|---|---|
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to human histone H3, di- or trimethylated at lysine 27 (H3K27me2/3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2608305 |
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and can undergo several types of post-translational modifications that influence cellular processes, including methylation or acetylation of lysine and arginine residues and phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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