Thermo Fisher Scientific H3K27ac Monoclonal Antibody, ChIP-Verified
H3K27ac 단일클론 항체로 ChIP 검증 완료. 인간 및 선충 시료에 반응하며 ICC/IF, ChIP, ChIP-Seq, pep-ELISA, CUT&RUN 등 다양한 응용에 사용 가능. 단백질 A 정제, 1 mg/mL 농도의 액상 형태로 제공. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | 1:500 | - |
| ChIP assay (ChIP) | 1–5 µg | View 2 publications |
| ChIP-sequencing (ChIP-Seq) | 3 µg | - |
| peptide-ELISA (pep-ELISA) | 1:3,000 | - |
| CUT&RUN (C&R) | 1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Nematode |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to human histone H3 acetylated at lysine 27 (H3K27ac). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608307 |
Product Specific Information
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Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones can alter chromatin structure or facilitate transcription factor binding. The N-terminal tail of histone H3 can undergo methylation, acetylation, and phosphorylation, influencing various cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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