Thermo Fisher Scientific Human R-Spondin 1 (aa 1-146) His-tag Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• Immunohistochemistry (IHC): Assay-dependent
• ELISA: Assay-dependent
• Functional Assay: Assay-dependent

Product Specifications

Activity

1. Measured by its ability to bind recombinant mouse CD36 in a functional ELISA.
2. Measured by its ability to induce activation of beta-catenin response in a Topflash Luciferase assay using HEK293T cells.
   • ED50 typically 0.1–0.5 µg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.

Product Specific Information

Reconstitution:
Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower.
It is recommended that the protein be aliquoted and used as soon as possible.
Store aliquots under sterile conditions at -20°C.
Avoid repeated freeze-thaw cycles.

Target Information

This gene encodes a secreted activator protein belonging to the R-spondin family, containing two cysteine-rich furin-like domains and one thrombospondin type 1 domain.
In mice, the protein promotes rapid crypt cell proliferation and enhances intestinal epithelial healing, providing protection against chemotherapy-induced damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.