Thermo Fisher Scientific Human R-Spondin 1 (aa 1-146) His-tag Recombinant Protein
Recombinant human R-Spondin 1 protein (aa 1-146) with C-terminal His-tag for functional assays. Expressed in HEK293 cells, >98% purity, endotoxin <1.0 EU/µg. Active in Wnt signaling and CD36 binding assays. Lyophilized form for research use only.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | HEK293 cells |
| Amino Acid Sequence | Human RSPO1, amino acids Met1-Ala146 (Accession # NP_001033722.1) with a C-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 14 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE |
| Endotoxin Concentration | <1.0 EU/µg |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Activity
- Measured by its ability to bind recombinant mouse CD36 in a functional ELISA.
- Measured by its ability to induce activation of beta-catenin response in a Topflash Luciferase assay using HEK293T cells.
- ED50 typically 0.1–0.5 µg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
Product Specific Information
Reconstitution:
Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower.
It is recommended that the protein be aliquoted and used as soon as possible.
Store aliquots under sterile conditions at -20°C.
Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a secreted activator protein belonging to the R-spondin family, containing two cysteine-rich furin-like domains and one thrombospondin type 1 domain.
In mice, the protein promotes rapid crypt cell proliferation and enhances intestinal epithelial healing, providing protection against chemotherapy-induced damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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